NNB Nutrition Announces Exclusive Distribution of RAMPS Dileucine Peptide

NNB Nutrition: We Create Ingredients

On May 9, 2024, NNB Nutrition announced that they have become the sole distributor of the new cutting edge RAMPS™ dileucine peptide supplement ingredient.[1] This is a patent-pending leucine dipeptide molecule that can outperform its branched-chain amino acid precursor, leucine, in stimulating muscle protein synthesis (MPS).

NNB is proud to announce that the initial exclusivity period for dileucine is over, and open distribution of the ingredient has begun.

NNB Nutrition RAMPS Dileucine

NNB Nutrition has announced open distribution of RAMPS Dileucine, after an exclusive period with MuscleTech in their Peptide 185 supplement.

What is Dileucine?

Most readers are familiar with leucine, the branched-chain amino acid (BCAA) that’s a key regulator of the mTOR pathway, which is critical for activating muscle protein synthesis in an effort to build skeletal muscle.[2-5]

Dileucine is a peptide bond of two leucine molecules,[6] and research shows that various peptide metabolites are responsible for a great deal of the anabolic effects of BCAAs.[6] More peptides in circulation generally means more efficient muscle production.

RAMPS promotes the following benefits, with research shown below in the next section:

  • 60% greater muscle protein synthesis activation versus leucine
  • 90% more leucine in the muscle within 30 minutes of consumption
  • Increased muscle protein synthesis without exercise* *(we don’t recommend avoiding exercise, but note for injuries and rest days)

Leucine vs. Dileucine in research

In a double-blind, randomized, crossover study published in 2021, researchers tested healthy young men, giving them 2 grams of leucine or 2 grams of dileucine peptide (the morning after they had all received identical dinners and had been abstaining from exercise), then measured numerous markers.[7]

As expected, the researchers saw that dileucine supplementation raised dileucine blood levels more than leucine. But what was more interesting was that they also saw a significantly greater fractional synthesis rate (FSR) in the dileucine group, whereas there was no significant difference in muscle breakdown.[7]

RAMPS Dileucine Fractional Synthesis Rate

The big story with dileucine is how it can increase fractional synthesis rate (FSR) significantly more than an equivalent amount of standard leucine.[7]

This means that dileucine’s net anabolic effect was greater than leucine’s. Dileucine actually increased MPS by about 160% compared to baseline, and about 60% more than leucine![7]

ISSN 2023 Presentation: 2g Dileucine vs. Leucine in Resistance-Trained Males

Effects of 10-weeks of Dileucine Supplementation on Athletic Performance

Effects of 10-weeks of Dileucine supplementation on athletic performance[8]

Another study was presented at the 2023 ISSN (International Society of Sports Nutrition) conference, where 32 resistance-trained males (average age 28) were recruited and given either 2 grams of dileucine, 2 grams of leucine, or placebo (2 grams of resistant starch) for 10 weeks.[8,9]

They trained 4 times per week, hitting every major muscle group with 3-4 sets at 6-10 reps.[9,10] The researchers found that dileucine improved maximum lower body strength and number of reps until failure more than leucine or placebo.[8]

University of Toronto: 2g Dileucine + 1g Leucine vs. 6g 2:1:1 BCAA vs. Collagen

A third randomized, double-blind, crossover study, which has been presented in Toronto but not yet published, used isotope tracking to show that dileucine increased leucine retention far greater than BCAA or collagen.[10] It also significantly reduced muscle breakdown in response to a whole-body resistance training regimen, whereas BCAA did not.[10]

Dileucine - University of Toronto Poster Presentation

In this poster presentation, dileucine significantly reduced muscle breakdown in response to whole-body resistance training, while BCAA did not.[10]

PricePlow will be first to provide coverage when these poster presentations are published in peer-reviewed journals — these are just a hint of what’s to come.

The History of RAMPS Dileucine Peptide

We first heard about dileucine on Episode #078 with Ralf Jaeger on the PricePlow Podcast, who mentioned some upcoming excitement about the ingredient at the 34:50 mark in the episode. Dr. Jaeger is with Ingenious Ingredients (ING2), who developed and patented the ingredient.

The body is “lazy”… but not when it comes to making dileucine

What struck our attention in that podcast is when he mentioned that the body is generally “lazy” with its energy, and never goes out of its way to do something unless there’s a very good biological reason. Yet it does go out of its way to form dileucine from leucine, which leads us to strongly believe that there is something incredibly important to the molecule.

MuscleTech Peptide 185 on the PricePlow Podcast

MuscleTech Peptide 185: Dileucine with Raza Bashir and Shawn Wells

Raza Bashir of MuscleTech and Shawn Wells of Ingenious Ingredients join the PricePlow Podcast for Episode #123 to discuss the new dileucine ingredient in Peptide 185!

MuscleTech was then given an exclusive first-to-market opportunity with the ingredient, producing their Peptide 185 supplement with incredible fan-fare and repeated sell-outs. Peptide 185 is labeled as DL-185™ dileucine (L-leucyl-L-leucine monohydrate), which is branding that they plan to maintain.

One of the best discussions on Peptide 185 and RAMPS dileucine is again on the PricePlow Podcast, this time in Episode #123 with MuscleTech’s Raza Bashir and NNB’s Shawn Wells, who give an incredible breakdown of the ingredient, its mechanisms, and the excitement surrounding it.

Iovate/MuscleTech confirmed that Peptide 185 will remain on the market, but with NNB’s announcement, Iovate’s exclusive period is over, and NNB now has open distribution.

You can read NNB Nutrition’s official press release for more information.[1] We have a few great formulation ideas for RAMPS, so contact us if you’d like to consult in forming some novel uses.

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About the Author: Mike Roberto

Mike Roberto

Mike Roberto is a research scientist and water sports athlete who founded PricePlow. He is an n=1 diet experimenter with extensive experience in supplementation and dietary modification, whose personal expertise stems from several experiments done on himself while sharing lab tests.

Mike's goal is to bridge the gap between nutritional research scientists and non-academics who seek to better their health in a system that has catastrophically failed the public. Mike is currently experimenting with a low Vitamin A diet.

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References

  1. NNB Nutrition. “Dileucine Press Release”. 09 May 2024. https://www.nnbnutrition.com/dileucine-press-release/
  2. Wilkinson, D J et al. “Effects of leucine and its metabolite β-hydroxy-β-methylbutyrate on human skeletal muscle protein metabolism.” The Journal of physiology vol. 591,11 (2013): 2911-23. doi:10.1113/jphysiol.2013.253203; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690694/
  3. Anthony, Joshua C., et al. “Signaling Pathways Involved in Translational Control of Protein Synthesis in Skeletal Muscle by Leucine.” The Journal of Nutrition, vol. 131, no. 3, Mar. 2001, pp. 856S860S, doi:10.1093/jn/131.3.856s. https://www.sciencedirect.com/science/article/pii/S0022316622147565
  4. Ham, Daniel J., et al. “Leucine as a Treatment for Muscle Wasting: A Critical Review.” Clinical Nutrition, vol. 33, no. 6, Dec. 2014, pp. 937–945, doi:10.1016/j.clnu.2014.09.016. https://pubmed.ncbi.nlm.nih.gov/25444557/
  5. A, Mero. “Leucine Supplementation and Intensive Training.” Sports Medicine (Auckland, N.Z.), 1 June 1999, doi:10.2165/00007256-199927060-00001. https://pubmed.ncbi.nlm.nih.gov/10418071/
  6. Morifuji, Masashi et al. “Comparison of different sources and degrees of hydrolysis of dietary protein: effect on plasma amino acids, dipeptides, and insulin responses in human subjects.” Journal of agricultural and food chemistry vol. 58,15 (2010): 8788-97. doi:10.1021/jf101912n. https://pubs.acs.org/doi/10.1021/jf101912n
  7. Paulussen, Kevin J.M., et al. “Dileucine Ingestion Is More Effective than Leucine in Stimulating Muscle Protein Turnover in Young Males: A Double Blind Randomized Controlled Trial.” Journal of Applied Physiology, vol. 131, no. 3, 1 Sept. 2021, pp. 1111–1122, doi:10.1152/japplphysiol.00295.2021. https://journals.physiology.org/doi/full/10.1152/japplphysiol.00295.2021
  8. Hagele, Anthony, et al. “Effects of 10 Weeks of Dileucine Supplementation on Athletic Performance”. Proceedings of the Twentieth International Society of Sports Nutrition (ISSN) Conference and Expo. Journal of the International Society of Sports Nutrition vol. 20,sup2 (2023): 2235311. doi:10.1080/15502783.2023.2235311. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388812/ (Poster Presentation PDF)
  9. Krieger, Joesi, et al. “Effects of 10 Weeks of Dileucine Supplementation on Body Composition in Resistance Trained Males”. Proceedings of the Twentieth International Society of Sports Nutrition (ISSN) Conference and Expo. Journal of the International Society of Sports Nutrition vol. 20,sup2 (2023): 2235311. doi:10.1080/15502783.2023.2235311. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388812/
  10. Aguilera, Jonathan, et al. “Dileucine-enriched essential amino acids support greater whole-body anabolism than branched chain amino acids and collagen hydrolysate after resistance exercise in recreationally active adults”. University of Toronto Poster Presentation. Retrieved January 2024. https://blog.priceplow.com/wp-content/uploads/dileucine-university-of-toronto-poster-presentation.pdf

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