In the supplement industry, having a single clinical study backing an ingredient is good. But having two separate clinical studies completed in the same year? That's validation.
Second Zynamite S study validates first findings. 100mg delivers 25% faster processing, 24% better mood, 26% less fatigue in 30 minutes. 88 students, non-stimulant, effects last hours. This is how you build clinical evidence.
PLT Health Solutions and their innovation partner Nektium Pharma recently announced results from their second clinical trial on Zynamite S,[1] a water-soluble mango leaf extract standardized to ≥60% mangiferin.
This new study, conducted on 88 healthy university students, confirms what the first study demonstrated: a single 100mg dose delivers measurable cognitive benefits that users can actually feel within 30 minutes.[1] The results are impressive, and they're also timely, given the race for immediately-experiential ingredients in beverages.
Compared to placebo, participants taking Zynamite S experienced 25% faster information processing, 24% improved mood, 26% reduced fatigue, and over 21% better performance on complex tasks. More than just subtle shifts in lab measurements, these are substantial improvements in real-world cognitive functions that matter for students, professionals, athletes, and anyone seeking sharper mental performance without stimulants.
This article brings a quick recap on Zynamite, then gets into the new study announced by PLT Health. Before going further, sign up for future Zynamite news from PLT, and then let's get into it:
Subscribe to PricePlow's Newsletter and Alerts on These Topics
What is Zynamite? Quick Refresher
For those new to this ingredient, Zynamite is a patented, proprietary extract from mango leaves (Mangifera indica) standardized to contain at least 60% mangiferin. Unlike caffeine and other stimulants, Zynamite delivers mental energy enhancement without affecting heart rate or blood pressure.
The Zynamite family now includes three distinct formulations:
- Zynamite - The original extract used primarily in capsules, tablets, and powder formulations
- Zynamite PX - Combines Zynamite with quercetin from Sophora japonica, formulated specifically for sports performance and recovery
- Zynamite S - The newest innovation: a water-soluble version designed for beverages and applications where solubility, neutral taste, and heat stability matter
According to PLT Health, Zynamite S offers approximately 3x greater bioavailability than standard Zynamite, allowing for lower effective doses while maintaining full efficacy. This makes it ideal for ready-to-drink beverages, shots, gummies, and sachets where traditional botanical extracts present formulation challenges.
The Second Zynamite S Study
This second clinical trial was designed to assess the rapid cognitive effects of a single 100mg dose of Zynamite S on healthy young adults. The study featured rigorous methodology that meets gold-standard requirements for supplement research:[1]
PLT Health Solutions' Zynamite S research shows impressive cognitive benefits. This water-soluble mango leaf extract improves processing speed, cognitive flexibility, and mood with effects lasting up to 5 hours after just one dose.
Study Design and Parameters:
- Participants: 88 healthy university students
- Design: Randomized, double-blind, placebo-controlled crossover with a 7-day washout period between testing conditions
- Intervention: Single 100mg dose of Zynamite S versus matching placebo
- Assessment Timeline: Baseline, 30 minutes, 3 hours, and 5 hours post-consumption
- Cognitive Tests Administered:
- Trail Making Test A (information processing speed)
- Trail Making Test B (cognitive flexibility and complex task handling)
- Stroop Color-Word Test (selective attention and inhibitory control)
- Digit Symbol Substitution Test (attention, working memory, and processing speed)
- Profile of Mood States (POMS) Test (mood assessment including total mood disturbances, tension, and fatigue)
The crossover design means each participant served as their own control, experiencing both the Zynamite S and placebo conditions in randomized order. This strengthens the study's internal validity by controlling for individual differences in baseline cognitive performance.
Study Results: Six Key Cognitive Enhancements
The results from this second study reinforce what makes Zynamite S so unique: broad-spectrum cognitive enhancement that starts working quickly and lasts for hours.[1]
-
Improved Mood Starting at 30 Minutes
Zynamite is PLT Health's Next-Gen Brain and Body Support Ingredient, harnessing the power of ≥60% mangiferin from mango leaf extract
Participants taking Zynamite S experienced significantly improved mood compared to placebo, with benefits appearing at the earliest measurement point (30 minutes) and continuing throughout the 5-hour testing period. By the final assessment, Zynamite S delivered a 24.1% improvement in Total Mood Disturbances as measured by the Profile of Mood States test.
According to Joe Weiss, Global Head of Brand Management for PLT Health Solutions, "This newest study of Zynamite S builds upon Nektium's previous research demonstrating efficacy in what scientists agree are the core components of 'mental energy': improved cognitive performance, cognitive adaptability, enhanced mood, and reduced fatigue. These results are shown to occur within minutes to hours. Zynamite S has the potential to redefine what we mean by mental energy in consumer products."
Mood enhancement matters for cognitive performance. When you feel clearer and more positive, you're better equipped to tackle challenging mental tasks with sustained focus.
-
Faster Information Processing After One Dose
Trail Making Test A measures processing speed by having participants connect numbered circles in sequence as quickly as possible. Zynamite S improved performance on this test by 25.4% compared to placebo, demonstrating faster mental processing.
In practical terms, this means connecting information more quickly, transitioning between tasks more efficiently, and responding to stimuli with less lag time. These improvements showed statistical significance and persisted across multiple time points.
-
Enhanced Attention and Working Memory
Are you ready for a shake-up in the beverage space? PLT Health Solutions has worked hard to create several beverage-stable ingredients, so that we can avoid all of the me-too RTD formulas.
The Digit Symbol Substitution Test evaluates attention, working memory, and processing speed simultaneously by requiring participants to match symbols to numbers according to a key, working as quickly and accurately as possible.
Participants taking Zynamite S improved their performance on this test by 17% at the 5-hour mark, with significant differences versus placebo emerging at 3 hours and continuing through the final assessment.
Working memory is critical for holding and manipulating information during complex cognitive tasks. Improvements here translate to better performance on activities requiring sustained concentration and mental flexibility.
-
Improved Mental Flexibility and Complex Task Handling
Trail Making Test B assesses cognitive flexibility and executive function by requiring participants to alternate between numbers and letters in ascending sequence (1-A-2-B-3-C, etc.). This task demands more complex mental operations than simply connecting numbers in order.
Zynamite S delivered a greater than 21.1% improvement in performance on this test, demonstrating enhanced ability to manage competing demands and switch between different types of information efficiently.
Executive function improvements like these are valuable for anyone facing complex problem-solving, multitasking, or decision-making under pressure.
-
Reduced Fatigue in 30 Minutes
Fatigue reduction appeared rapidly after Zynamite S consumption. At the earliest measurement point (30 minutes), participants experienced 26% less fatigue compared to placebo. Tension also decreased significantly within the same timeframe.
This rapid onset contributes to what Dr. Laura López-Ríos, Product Development Director at Nektium, describes as the "experiential" quality of Zynamite S. Users don't need to wait days or weeks to notice benefits. The effects are apparent within the first 30 minutes and build from there.
-
Improved Selective Attention
The Stroop Color-Word Test evaluates selective attention and inhibitory control by presenting color words printed in conflicting ink colors (the word "RED" printed in blue ink, for example). Participants must name the ink color while inhibiting the automatic response to read the word.
Zynamite S improved performance on this test by 4.7% compared to placebo. While this percentage may seem modest, inhibitory control is fundamental to managing distractions and maintaining focus in complex environments. Small improvements in this domain can have outsized practical impact.
Comparing the Two Studies
New clinical data on Zynamite S shows cognitive and mood benefits within 30 minutes, lasting 3-5 hours. PLT Health Solutions presented research at SupplySide Global 2025 and featured it in CREATEA Mighty Mango. Water-soluble, non-stimulant mental energy.
Having two separate clinical studies on Zynamite S within a single year provides a valuable perspective on the consistency and reliability of its effects.
The first study, which we covered earlier this year, tested 120 healthy university students and examined both 100mg and 150mg doses of Zynamite S. The second study focused exclusively on the 100mg dose with 88 participants.
Consistent findings across both studies:
- Rapid onset of cognitive benefits (within 30 minutes)
- Improved information processing speed
- Enhanced cognitive flexibility and complex task performance
- Better attention and working memory
- Mood improvements and fatigue reduction
- Effects persisting for multiple hours after a single dose
The convergence of results between these two trials strengthens confidence in Zynamite S as a reliable cognitive enhancer. When different research teams obtain similar findings using the same ingredient, it reduces the likelihood that positive results occurred by chance or due to methodological quirks specific to one study.
Dr. López-Ríos emphasized this point: "The results of this study certainly demonstrate that using Zynamite S is 'experiential.' This second study on Zynamite S within a single year validates and expands upon our previous findings and demonstrates our commitment to better understanding and communicating the benefits that are possible with our ingredients."
Why A Second Study Matters
CREATEA Mighty Mango breaks new ground with stable creatine in RTD format plus Zynamite S mango leaf extract for mental energy. This natural, clean-label concept beverage showcases what's possible when industry leaders collaborate. Sample it at SupplySide Global 2025!
In the supplement industry, many ingredients rely on a single clinical trial to support their marketing claims. While one well-designed study is valuable, having multiple studies provides substantially stronger evidence.
Replication addresses several critical concerns:
- Confirmation of effects: The same results appearing in different samples increases confidence that the effects are real and reproducible
- Generalizability: Testing different participant groups helps establish that benefits extend beyond one specific population
- Scientific rigor: Multiple studies demonstrate ongoing research commitment rather than one-and-done evidence gathering
- Risk reduction for formulators: Brands can formulate with greater confidence when multiple studies support an ingredient's efficacy
Additionally for brands up north, Health Canada often requires more than one clinical study before allowing for claims.
For formulators evaluating cognitive ingredients, the difference between single-study support and multi-study validation is significant. Zynamite S now offers the latter, positioning it as a serious option backed by robust clinical evidence.
The fact that both studies were completed within a single year also signals Nektium and PLT's commitment to building a comprehensive research foundation for this ingredient. This isn't an ingredient with decades-old research that hasn't been updated. This is active, ongoing investigation into optimal dosing, timing, and effects.
Mechanism of Action: Non-Stimulant Mental Energy
PLT Health Solutions: Growth Through Innovation
What makes Zynamite's cognitive benefits valuable is how they're achieved. Unlike caffeine, which works by blocking adenosine receptors and increasing arousal, Zynamite operates through a different mechanism, which is strongly suggested by previous researchers to be catechol-O-methyltransferase (COMT) inhibition.
COMT is an enzyme responsible for breaking down catecholamine neurotransmitters like dopamine, especially in the prefrontal cortex. By inhibiting this enzyme, mangiferin may help maintain higher dopamine levels in brain regions associated with executive function, working memory, and attention.
Crucially, Zynamite achieves these cognitive effects without cardiovascular stimulation. Previous studies have confirmed that Zynamite does not significantly affect heart rate, blood pressure, or heart rate variability. For consumers sensitive to stimulants or those seeking cognitive enhancement without physiological arousal, this distinction matters.
For a deeper exploration of Zynamite's mechanisms, see our main Zynamite article.
The Water-Soluble Advantage
Beyond the cognitive benefits themselves, what makes Zynamite S has important formulation characteristics. Traditional botanical extracts often present challenges in beverages: poor solubility, strong flavors, instability under heat processing, and cloudiness in solution.
Zynamite S was specifically engineered to address these barriers:
- Enhanced water solubility for clear solutions in ready-to-drink beverages
- Neutral taste profile that doesn't require extensive flavor masking
- Heat stability for pasteurization and other thermal processing
- More transparency in solution for clean-looking products
- Approximately 3x greater bioavailability than standard Zynamite
These advantages extend Zynamite's reach into PLT Health's beverage-stable platform, making it viable for ready-to-drink beverages, shots, gummies, sachets, and powder formulations where mixability and taste matter.
As an example of Zynamite S in action, PLT featured it in the CREATEA Mighty Mango concept beverage that debuted at SupplySide Global 2025. This collaboration combined Zynamite S with beverage-stable creatine, natural caffeine, and other performance ingredients, demonstrating the ingredient's versatility in multi-functional formulations.
At 100mg, the lower effective dose for Zynamite S (compared to standard Zynamite) gives it more flexibility. This creates opportunities for formulators to include meaningful amounts of additional ingredients without exceeding serving size constraints.
Target applications include:
- Pre-workout supplements (cognitive and physical performance)
- Productivity and focus beverages (mental energy without stimulation)
- Energy drinks seeking non-caffeine cognitive benefits
- Gaming and esports formulations (attention and reaction time)
- Daily wellness beverages (sustained mental clarity)
The fast onset and experiential nature of Zynamite S's benefits make it especially suitable for products where users expect to feel effects quickly.
Conclusion: Validated Through Replication
The completion of a second clinical study on Zynamite S within a single year represents a commitment to building serious scientific support for an ingredient that delivers meaningful, measurable cognitive benefits, and does so quickly.
Industry leaders from PLT Health Solutions, Glanbia Nutritionals, NNB Nutrition, NutriScience Innovations, and LaCore Nutraceuticals discuss the collaborative innovation behind CREATEA Mighty Mango at SupplySide Global 2025 on Episode #189 of the PricePlow Podcast.
With both studies confirming rapid onset (benefits starting at 30 minutes), substantial improvements across multiple cognitive domains (processing speed, flexibility, attention, mood), and sustained effects lasting several hours from a single dose, Zynamite S has established itself as a serious player in the cognitive enhancement space.
For formulators, the combination of strong clinical backing, water-soluble functionality, neutral taste, and experiential benefits creates compelling opportunities across multiple product categories. For consumers, it offers non-stimulant mental energy that they can actually feel working.
As the demand for cognitive enhancement ingredients continues growing, especially among those seeking alternatives to high-dose caffeine, Zynamite S is well-positioned to meet that need. The ingredient's versatility in beverages, combined with its clinical validation, suggests we'll see increasing adoption in the coming months.
This article will be updated when the full journal publication of this second Zynamite S study becomes available.
Comments and Discussion (Powered by the PricePlow Forum)